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In enzymology, a polynucleotide 5'-hydroxyl-kinase () is an enzyme that catalyzes the chemical reaction :ATP + 5'-dephospho-DNA ADP + 5'-phospho-DNA Thus, the two substrates of this enzyme are ATP and 5'-dephospho-DNA, whereas its two products are ADP and 5'-phospho-DNA. Polynucleotide kinase is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ATP to the free hydroxyl end of the 5' DNA or RNA. The resulting product could be used to end-label DNA or RNA, or in a ligation reaction. == Nomenclature == This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:5'-dephosphopolynucleotide 5'-phosphotransferase. Other names in common use include: *ATP:5'-dephosphopolynucleotide 5'-phosphatase * PNK * polynucleotide 5'-hydroxyl kinase (phosphorylating), * 5'-hydroxyl polynucleotide kinase, * 5'-hydroxyl polyribonucleotide kinase, * 5'-hydroxyl RNA kinase, * DNA 5'-hydroxyl kinase, * DNA kinase, * polynucleotide kinase, and * polynucleotide 5'-hydroxy-kinase. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Polynucleotide 5'-hydroxyl-kinase」の詳細全文を読む スポンサード リンク
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